A review of the methods used to determine the target site or the mechanism of action of essential oils and their components against fungi

Abstract Essential oils (EOs) are complex mixtures of compounds derived from plants that exhibit antimicrobial activity. Several studies have demonstrated their antifungal activity in food matrices or vitro via vapor phase direct addition. Recently, researchers focusing on elucidating the target site mechanism action various EOs. Past research has suggested evidence how EOs act fungal cells assays assessed cell wall alterations gene expression modifications. However, no previous reports summarized most methods for finding Therefore, this review presents and used to discover against cells. Researchers commonly analyze plasma membrane integrity using techniques as well changes morphology. Meanwhile, quantification mitochondrial enzymes, ROS species, less assayed.